Materials and reagents SHLI and its two intermediate fractions (F1, the extract of Scutellariae Radix; F2, the extract of Lonicerae Japonicae Flos and Fructus Forsythiae) were prepared by Duoduo Pharmaceutical Co., Ltd. (Jiamusi, Heilongjiang, China) according to the Chinese Pharmacopoeia1. Compound 48/80 (C48/80), propranolol, CV3988, triprolidine, globulins, cimetidine, and SB290157 were purchased from Sigma-Aldrich (St
1. Victora CG, Bahl R, Barros AJD, Franca GVA, Horton S, Krasevec J, et al. Breastfeeding in the 21st century: Epidemiology, mechanisms, and lifelong effect. Lancet. 2016;387:475–90. 2. Heinrich J. Modulation of allergy risk by breast feeding. Curr Opin Clin Nutr Metab Care. 2017;20:217–21. 3. Lodge CJ, Tan DJ, Lau M, Dai X, Tham R,
Women who work as cleaners or regularly use cleaning sprays or other cleaning products at home appear to experience a greater decline in lung function over time than women who do not clean, according to new research published online in the American Thoracic Society’s American Journal of Respiratory and Critical Care Medicine. In “Cleaning at
New research findings could turn perceptions of the already despised bed bug from nuisance pest into medically important threat. A study from North Carolina State University shows that histamine levels are substantially higher in homes infested by bed bugs than in pest-free homes, and that these histamine levels persist for months — even if the
Marine shipping fuels will get a whole lot cleaner in 2020 when a regulation by the International Maritime Organization (IMO) requires fuels to contain 80-86 percent less sulphur. This is the most significant improvement in global fuel standards for the shipping industry in 100 years, intended to achieve significant health benefits on a global scale.
A real milk allergy occurs in about three to five percent of European children and more rarely in adults. The disease is different from lactose intolerance, in which a lack of the enzyme lactase results in the inability to properly break down lactose, a sugar found in milk products. In the case of a milk
Houston Methodist researchers have a new explanation for what causes the lungs’ airways to close during asthma attacks that could change the lives of the 300 million people worldwide who suffer from asthma. The discovery holds promise for developing a new class of drugs that is radically different from the steroids currently used to treat
How healthy is your almond milk really? It may taste good and may not cause you any of the unpleasant reactions caused by cow’s milk. But though plant-based milk beverages of this kind have been on the market for a couple of decades and are advertised as being healthy and wholesome for those who are
Researchers have found a new mechanism in which an antibody can prevent allergic reactions in a broad range of patients. The breakthrough could pave the way for a far more effective allergy medicine. There was great excitement in the laboratory when researchers from Aarhus University recently discovered the unique mechanisms of an antibody that blocks
Our results indicate that rural (i.e. non-built) residential environments are associated with lower risk of owner-reported allergic symptoms in dogs. Moreover, several factors related to rural living, such as living in outdoor facilities and having regular contact with other animals (other pets and/or farm animals), are related to lower risk of allergic symptoms (i.e. high
1. Eder, W., Ege, M. J. & von Mutius, E. The asthma epidemic. N Engl J Med 355, 2226–2235 (2006). 2. Sicherer, S. H. & Sampson, H. A. Food allergy: Epidemiology, pathogenesis, diagnosis, and treatment. J Allergy Clin Immunol 133, 291–307; quiz 308 (2014). 3. Prescott, S. & Allen, K. J. Food allergy: riding the
Severe eczema, also known as atopic dermatitis, is a chronic inflammatory skin condition that is driven by an allergic reaction. In their latest study, researchers at La Jolla Institute reveal an important player that promotes skin inflammation in atopic dermatitis and the characteristic thickening of the skin. Their work, published in the January 16, 2018,
When babies crawl, their movement across floors, especially carpeted surfaces, kicks up high levels of dirt, skin cells, bacteria, pollen, and fungal spores, a new study has found. The infants inhale a dose of bio bits in their lungs that is four times (per kilogram of body mass) what an adult would breathe walking across
1. Renz, H., Brandtzaeg, P. & Hornef, M. The impact of perinatal immune development on mucosal homeostasis and chronic inflammation. Nat. Rev. Immunol. 12, 9–23 (2011). 2. Cao, S., Feehley, T. J. & Nagler, C. R. The role of commensal bacteria in the regulation of sensitization to food allergens. FEBS Lett. 588, 4258–4266 (2014). 3.
Crystallization The 026 sdab:IgE Fc complex was concentrated to 5 mg ml−1 in 20 mM HEPES, 50 mM NaCl, pH 7.2, and crystallized by vapor diffusion in sitting drops formed by mixing protein and reservoir solution containing 0.1 M imidazole pH 7.0, 11–12% polyethylene glycol (PEG) 20,000 in the ratio 1:1. Crystals were cryocooled in liquid nitrogen after transfer to
Researchers from Universidad Politécnica de Madrid (UPM) find direct evidence that the ligands, compounds carried by allergens, are actively involved in the allergic sensitization phase. Researchers from Centre for Plant Biotechnology and Genomics (CBGP), a joint research centre of UPM and INIA, ha found that certain compounds carried by allergic proteins would be collaborating agents
A full understanding of the pathogenesis of CSU has yet to be achieved. In the present study, to gain better understanding of the role of cytokines in immunopathogenesis of CSU we aimed to determine whether CSU is associated with alterations in IL-17, IL-31, and IL-33. The results of our study showed that plasma levels of
At least a third of reactions in children with food-induced anaphylaxis to a known allergen occur under adult supervision, according to a new study led by AllerGen researchers in Quebec, Ontario and Alberta. The findings, published in the November issue of Pediatric Allergy and Immunology, reveal that inadvertent exposures to a known food allergen in
Antibodies Mouse monoclonal antibody against IL-33 (clone Nessy-1) (#ALX-804-840-C100) was purchased from Enzo. Rabbit polyclonal antibodies against KRT5 (#ab24647) and Ki-67 (#ab15580) were purchased from Abcam (Abcam, Cambridge, MA). Rabbit polyclonal antibody against KRT14 (#PRB-155P) was purchased from Covance (Covance, Princeton, NJ). Rabbit polyclonal antibody against KRT4 (#HPA034881) was purchased from Sigma (Sigma-Aldrich Corp, St.
Clinical characteristics of the peanut allergic cohort Twenty-one children with suspected peanut allergy completed randomized, double-blind, placebo-controlled oral food challenges to peanut, performed according to a modified AAAAI/EAACI PRACTALL protocol8, 9. Under close medical supervision with blinding of subjects and staff, subjects ingested incremental amounts of peanut at 20 min intervals until an allergic reaction occurred
A new study in EBioMedicine by researchers at Karolinska Institutet in Sweden and the Medical University of Vienna, Austria suggests that immune response in early childhood to a handful of allergen molecules can predict the onset of allergic rhinitis and asthma in adolescence. These findings could accelerate the development of preventive strategies and novel treatments
A recent review paper outlines the many risk factors for asthma including those that are psychological, social, and lifestyle-related. The most common chronic illness that affects children in the United States is asthma. Approximately 14.5% of children have this disease and it adversely affects their physical and psychosocial health. Many environmental, psychological, and social factors
Publishing online this week in Cell Host & Microbe, researchers at Johns Hopkins report the discovery of a key underlying immune mechanism that explains why to how our skin becomes inflamed from conditions such as atopic dermatitis, more commonly known as eczema. Toxin-producing bacteria on the surface of our skin induces a protein that causes
Scientists are finding more evidence that commonly prescribed antidepressants aren’t effective in people battling both depression and a chronic medical disease, raising a critical question of whether doctors should enact widespread changes in how they treat millions of depressed Americans. A new study published in the Journal of the American Medical Association found depressed patients
A phase II trial of Tazepelumab – an asthma medication developed by pharmaceutical companies MedImmune and Amgen – yielded exciting results for many patients with uncontrolled asthma. Over 315 million people across the globe are affected by asthma – a long-term inflammatory disease of the lungs. Most cases can be controlled by using glucocorticoids administered
1. Pinart, M. et al. Comorbidity of eczema, rhinitis, and asthma in IgE-sensitised and non-IgE-sensitised children in MeDALL: a population-based cohort study. Lancet Respir. Med. 2, 131–140 (2014). 2. Thomsen, S.F. et al. Findings on the atopic triad from a Danish twin registry. Int. J. Tuberc. Lung Dis. 10, 1268–1272 (2006). 3. van Beijsterveldt, C.E.
The skin prick test has several flaws, prompting researchers to investigate into alternative molecular allergy diagnostic tests. Skin prick tests involve pricking an extract of a suspected allergen onto the surface of the skin. Redness and swelling at the test site indicate a Type I allergy, which is caused by the antibody IgE. This is
Study design and quality control To identify genes involved in food allergy, we performed a GWAS in a discovery set of 523 food allergic cases and 2682 population-based controls from the German Heinz Nixdorff Recall Study (HNR)16. All cases were from the Genetics of Food Allergy Study (GOFA) in which the diagnosis of food allergy